BlokHen® Blocking Reagent
Our proprietary blocking reagent, BlokHen® is a fish serum-based immunoreagent designed for use with chicken antibodies. It already contains concentrated buffer and non-ionic detergent (tween-20), allowing simple dilution with water. BlokHen® can be used in Western Blots, Immunohistochemistry or Immunocytochemistry, Immunoprecipitation, or ELISA applications.
BlokHen® Blocking Reagent
Product Specific References for Applications and Species
- ELISA: Chicken | Human
- Immunocytochemistry: Canine | Human | Mouse | Rat
- Immunohistochemistry: Canine | Chicken | Drosophila | Human | Mouse | Rat
- Western Blot: Human | Mouse
- Additional Publications: Unspecified
| ELISA: Chicken | ||
| PMID | Dilution | Publication |
| 31275378 | 1:100 | Chavez-Cortez, E.G., et al. 2019. Production and evaluation of an avian IgY immunotoxin against CD133+ for treatment of carcinogenic stem cells in malignant glioma: IgY immunotoxin for the …. Journal of Oncology, 2563092. |
| 25131991 | 1.0% | Anji, A., et al. 2015. Expression of α‐subunit of α‐glucosidase II in adult mouse brain regions and selected organs. Journal of Neuroscience Research, 82-93. |
| ELISA: Human | ||
| PMID | Dilution | Publication |
| 30229864 | 1:100 | Smith, D.R.,, et al. 2019. Combinatorial lentiviral gene delivery of pro‐oligodendrogenic factors for improving myelination of regenerating axons after spinal cord injury. Biotechnology and Bioengineering, 155-167. |
| Immunocytochemistry: Canine | ||
| PMID | Dilution | Publication |
| 21277207 | 10% | Berg, Z., et al. 2011. Muscle satellite cells from GRMD dystrophic dogs are not phenotypically distinguishable from wild type satellite cells in ex vivo culture. Neuromuscular Disorders, 282-290. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 34452409 | 1:2000 | Funk, C., et al. 2021. The HSV1 Tail-Anchored Membrane Protein pUL34 Contains a Basic Motif That Supports Active Transport to the Inner Nuclear Membrane Prior to Formation of the Nuclear Egress Complex. Viruses, 1544. |
| 30301799 | 10% | Katsumura, K.R., et al. 2018. Human leukemia mutations corrupt but do not abrogate GATA-2 function. PNAS: USA, E10109-10118. |
| 28370412 | 1:10 | Kamalakar, A., et al. 2017. PTHrP (12‐48) Modulates the Bone Marrow Microenvironment and Suppresses Human Osteoclast Differentiation and Lifespan. Journal of Bone and Mineral Research, 1421-1431. |
| 28351373 | 20% | Guan, F.H., et al. 2017. The antiproliferative ELF2 isoform, ELF2B, induces apoptosis in vitro and perturbs early lymphocytic development in vivo. Journal of Hematology and Oncology, 75. |
| 28319062 | 20% | Marshall, A.D., et al. 2017. CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic. Oncogene, 4100-4110. |
| 28142205 | 5% | Bajpai, V.K., et al. 2017. Reprogramming postnatal human epidermal keratinocytes toward functional neural crest fates. Stem Cells, 1402-1415. |
| 20106917 | 10% | Roller, R.J., et al. 2010. Analysis of a charge cluster mutation of herpes simplex virus type 1 UL34 and its extragenic suppressor suggests a novel interaction between pUL34 and pUL31 that …. Journal of Virology, 3921-3924. |
| 19934382 | not listed | Yuan, L., et al. 2010. A stretch of 17 amino acids in the prosaposin C terminus is critical for its binding to sortilin and targeting to lysosomes. The Journal of Histochemistry and Cytochemistry, 287-300. |
| 15140953 | 10% | Reynolds, A.E., et al. 2004. Conformational Changes in the Nuclear Lamina Induced by Herpes Simplex Virus Type 1 Require Genes UL31 and UL34. Journal of Virology, 5564-5575. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36214254 | 1:1000 | LaCombe, R., et al. 2022. EphA1 Receptor Tyrosine Kinase is Localized to the Nucleus in Rhabdomyosarcoma from Multiple Species. Biology Open, . |
| 26305592 | not listed | Garcia-Gonzalo, F.R., et al. 2015. Phosphoinositides regulate ciliary protein trafficking to modulate hedgehog signaling. Developmental Cell, 400-409. |
| 18371421 | 10% | Brack, A.S., et al. 2008. A temporal switch from notch to Wnt signaling in muscle stem cells is necessary for normal adult myogenesis. Cell Stem Cell, 50-59. |
| 18308302 | 10% | Capkovic, K.L., et al. 2008. Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation. Experimental Cell Research, 1553-1565. |
| 17652388 | 10% | Leach, N., et al. 2007. Emerin is hyperphosphorylated and redistributed in herpes simplex virus type 1-infected cells in a manner dependent on both UL34 and US3. Journal of Virology, 10792-10803. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 18759491 | not listed | Malarkey, E.B., et al. 2008. Water soluble single-walled carbon nanotubes inhibit stimulated endocytosis in neurons. Nano Letters, 3538-3542. |
| Immunohistochemistry: Canine | ||
| PMID | Dilution | Publication |
| 30520657 | 10% | Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273. |
| Immunohistochemistry: Chicken | ||
| PMID | Dilution | Publication |
| 19627987 | 10% | Beazley, K.E., et al. 2009. Developmental regulation of the nuclear ferritoid–ferritin complex of avian corneal epithelial cells: Roles of systemic factors and thyroxine. Experimental Eye Research, 854-862. |
| 12952943 | 1:5 | Hood, J.D., et al. 2003. Differential αv integrin–mediated Ras-ERK signaling during two pathways of angiogenesis. Journal of Cell Biology, 933-943. |
| Immunohistochemistry: Drosophila | ||
| PMID | Dilution | Publication |
| 30520657 | 10% | Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273. |
| 27017624 | 10% | Sanghavi, P., et al. 2016. Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber. Genetics, 415-432. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 30520657 | 10% | Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273. |
| 20150438 | 10% | Harigopal, M., et al. 2010. Multiplexed assessment of the Southwest Oncology Group-directed Intergroup Breast Cancer Trial S9313 by AQUA shows that both high and low levels of …. American Journal of Pathology, 1639-1647. |
| 20054338 | 10% | Liu, Y., et al. 2010. Regulation of the psoriatic chemokine CCL20 by E3 ligases Trim32 and Piasy in keratinocytes. Journal of Investigative Dermatology, 1384-1390. |
| 19862339 | 5% | Iragavarapu, S., et al. 2009. ETX1 is over-expressed in the glaucomatous trabecular meshwork. Molecular Vision, 2061-2067. |
| 19213873 | 1:10 | Ransford, G.A., et al. 2009. Pannexin 1 contributes to ATP release in airway epithelia. American Journal of Cellular and Molecular Biology, 525-534. |
| 18424768 | 20% | Kowalczuk, S., et al. 2008. A protein complex in the brush‐border membrane explains a Hartnup disorder allele. FASEB, 2880-2887. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36936074 | 10% | Xing, Y.L., et al. 2023. High-Efficiency Pharmacogenetic Ablation of Oligodendrocyte Progenitor Cells in the Adult Mouse CNS. Cell Reports Methods, 100414. |
| 36932080 | not listed | Berry, M.H., et al. 2023. A Melanopsin Ganglion Cell Subtype Forms a Dorsal Retinal Mosaic Projecting to the Supraoptic Nucleus. Nature Communications, 1492. |
| 36932080 | not listed | Berry, M.H., et al. 2023. A Melanopsin Ganglion Cell Subtype Forms a Dorsal Retinal Mosaic Projecting to the Supraoptic Nucleus. Nature Communications, 1492. |
| 36708949 | 7.5% | Nayak, S., et al. 2023. Chromatin landscape governing murine epidermal differentiation. The Journal of Investigative Dermatology, . |
| 36287038 | 1:500 | Zhang, W., et al. 2022. Bone Metastasis Initiation is Coupled with Bone Remodeling Through Osteogenic Differentiation of NG2+ Cells. Cancer Discovery, 474-495. |
| 35276096 | 1% | Gadomski, S., et al. 2022. A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise. Cell Stem Cell, 528-544. |
| 35192546 | 1% | Valet, C., et al. 2022. Sepsis promotes splenic production of a protective platelet pool with high CD40 ligand expression. The Journal of Clinical Investigation, e153920. |
| 35087060 | 1% | Fielding, C., et al. 2022. Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis. Nature Communication, 543. |
| 33338666 | not listed | Khalid, A.B., et al. 2021. GATA4 regulates mesenchymal stem cells via direct transcriptional regulation of the WNT signalosome. Bone, 115819. |
| 31994726 | 1:20 | Brooks, P.M., et al. 2020. Pou3f4‐expressing otic mesenchyme cells promote spiral ganglion neuron survival in the postnatal mouse cochlea. Journal of Comparative Neurology, 1967-1985. |
| 30520657 | 10% | Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273. |
| 29536590 | 10% | Harley, R.J., et al. 2018. Neuronal cell adhesion molecule (NrCAM) is expressed by sensory cells in the cochlea and is necessary for proper cochlear innervation and sensory domain …. Developmental Dynamics, 934-950. |
| 27760456 | 7.5% | Duverger, O., et al. 2017. DLX3‐dependent regulation of ion transporters and carbonic anhydrases is crucial for enamel mineralization. Journal of Bone Mineral Research, 641-653. |
| 27339393 | 10% | Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), enriched in ventral hippocampus neurons, is required for consolidation of social but not nonsocial memories in mice. Neuropsychopharmacology, 2920-2931. |
| 26962307 | not listed | Wang, P.R., et al. 2016. Cellular origins of regenerating nodules and malignancy in the FAH model of liver injury after bone marrow cell transplantation. Stem Cells International, 5791317. |
| 26302206 | 10% | Coate, T.M., et al. 2015. Neuropilin-2/Semaphorin-3F-mediated repulsion promotes inner hair cell innervation by spiral ganglion neurons. Elife, e07830. |
| 25319708 | 10% | Xing, Y.L., et al. 2014. Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination. Journal of Neuroscience, 14128-14146. |
| 25267791 | 7.5% | Dema, B., et al. 2014. Immunoglobulin E plays an immunoregulatory role in lupus. Journal of Experimental Medicine, 2159-2168. |
| 22351765 | 7.5% | Duverger, O., et al. 2012. Neural crest deletion of Dlx3 leads to major dentin defects through down-regulation of Dspp. Journal of Biological Chemistry, 12230-12240. |
| 21636576 | 20% | Bröer, A., et al. 2011. Impaired nutrient signaling and body weight control in a Na+ neutral amino acid cotransporter (Slc6a19)-deficient mouse. Journal of Biological Chemistry, 26638-26651. |
| 20151365 | 1% | White, R.E., et al. 2010. Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice. Journal of Comparative Neurology, 1370-1390. |
| 19711380 | not listed | Yee, C.L., et al. 2009. Arcuate nucleus expression of NKX2. 1 and DLX and lineages expressing these transcription factors in neuropeptide Y+, proopiomelanocortin+, and tyrosine …. Journal of Comparative Neurology, 37-50. |
| 19657021 | 1:10 | Liu, M.T., et al. 2009. 5-HT4 receptor-mediated neuroprotection and neurogenesis in the enteric nervous system of adult mice. Journal of Neuroscience, 9683-9699. |
| 12543640 | 1:10 | Zhang, Y., et al. 2003. Syndecan 4 modulates basic fibroblast growth factor (bFGF) signaling in vivo. American Journal of Physiology. Heart and Circulatory Physiology, H2078-H2082. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 29454014 | not listed | Gluck, M.J., et al. 2018. Detecting structural and inflammatory response after in vivo stretch injury in the rat median nerve via second harmonic generation. Journal of Neuroscience Methods, 68-80. |
| 23031825 | 10% | Coutts, D.J., et al. 2013. Embryonic-derived olfactory ensheathing cells remyelinate focal areas of spinal cord demyelination more efficiently than neonatal or adult-derived cells. Cell Transplantation, 1249-1261. |
| 22138179 | 10% | Dmitrieff, E.F., et al. 2012. Carotid body growth during chronic postnatal hyperoxia. Respiratory Physiology and Neurobiology, 193-203. |
| 21094282 | 10% | Dmitrieff, E.F., et al. 2011. Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats. Respiratory Physiology and Neurobiology, 220-227. |
| 17293479 | Jasmin, J.F., et al. 2007. Caveolin-1 deficiency increases cerebral ischemic injury. Circulation Research, 721-729. | |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 28370412 | 1:10 | Kamalakar, A., et al. 2017. PTHrP (12‐48) Modulates the Bone Marrow Microenvironment and Suppresses Human Osteoclast Differentiation and Lifespan. Journal of Bone and Mineral Research, 1421-1431. |
| 19101982 | 10% | Cieply, B., et al. 2009. Unique phenotype of hepatocellular cancers with exon‐3 mutations in beta‐catenin gene. Hepatology, 821-831. |
| 16920825 | 1:10 | Wills, E., et al. 2006. Herpes Simplex Virus 1 DNA Packaging Proteins Encoded by UL6, UL15, UL17, UL28, and UL33 Are Located on the External Surface of the Viral Capsid. Journal of Virology, 10894-10899. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 20685971 | 20% | Strumbos, J.G., et al. 2010. Fragile X mental retardation protein is required for rapid experience-dependent regulation of the potassium channel Kv3. 1b. Journal of Neuroscience, 10263-10271. |
| 17470426 | 10% | Hwang, J., et al. 2007. Role of Scarf and its binding target proteins in epidermal calcium homeostasis. Journal of Biological Chemistry, 18645-18653. |
| 10075978 | 10% | Schneider, T.J., et al. 1999. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. Journal of Experimental Medicine, 949-956. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 28884467 | Sundberg, S.C., et al. 2018. Cre‐expressing neurons in visual cortex of Ntsr1‐Cre GN220 mice are corticothalamic and are depolarized by acetylcholine. Journal of Comparative Neurology, 120-132. | |
| 21071039 | Lee, J.H., et al. 2011. Schwann cell-like remyelination following transplantation of human umbilical cord blood (hUCB)-derived mesenchymal stem cells in dogs with acute spinal cord injury. Journal of the Neurological Sciences, 86-96. | |
| 17439301 | Conboy, M.J., et al. 2007. High incidence of non-random template strand segregation and asymmetric fate determination in dividing stem cells and their progeny. PLoS Biology, e102. | |
| 17346701 | Loitto, V.M., et al. 2007. Filopodia are induced by aquaporin-9 expression. Experimental Cell Research, 1295-1306. | |
| 17295233 | Hudson, L.G., et al. 2007. Ultraviolet radiation stimulates expression of Snail family transcription factors in keratinocytes. Molecular Carcinogenesis, 257-268. | |
| 17200142 | Sigal, Y.J., et al. 2007. Cdc42 and ARP2/3-independent regulation of filopodia by an integral membrane lipid-phosphatase-related protein. Journal of Cell Science, 340-352. | |
| 16969078 | Hnasko, R., et al. 2006. PV-1 labels trans-cellular openings in mouse endothelial cells and is negatively regulated by VEGF. Cell Cycle, 2021-2028. | |
| 16513825 | Peluso, J.J., et al. 2006. Progesterone membrane receptor component 1 expression in the immature rat ovary and its role in mediating progesterone's antiapoptotic action. Endocrinology, 3133-3140. | |
| 16427676 | Bjerke, S.L., et al. 2006. Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress. Virology, 261-276. | |
| 16216560 | Held, P.K., et al. 2006. In vivo genetic selection of renal proximal tubules. Molecular Therapies, 49-58. | |
| 16154690 | Reiman, R., et al. 2005. Expression of C5a in the brain does not exacerbate experimental autoimmune encephalomyelitis. Neuroscience Newsletters, 134-138. | |
| 16153295 | Franklin, R.B., et al. 2005. hZIP1 zinc uptake transporter down regulation and zinc depletion in prostate cancer. Molecular Cancer, 32. | |
| 16037544 | Liu, M., et al. 2005. Expression and function of 5-HT4 receptors in the mouse enteric nervous system. American Journal of Physiology. Gastrointestinal and Liver Physiology, G1148-G1163. | |
| 15951442 | Poliakov, E., et al. 2005. Key role of conserved histidines in recombinant mouse β-carotene 15, 15′-monooxygenase-1 activity. Journal of Biological Chemistry, 29217-29223. | |
| 15914599 | Lu, X., et al. 2005. Identification of novel murine-and human-specific RPGRIP1 splice variants with distinct expression profiles and subcellular localization. Investigative Opthalmology and Visual Science, 1882-1890. | |
| 15862180 | Bhattacharya, S.K., et al. 2005. Cochlin deposits in the trabecular meshwork of the glaucomatous DBA/2J mouse. Experimental Eye Research, 741-744. | |
| 15814896 | Peluso, J.J., et al. 2005. Expression and function of PAIRBP1 within gonadotropin-primed immature rat ovaries: PAIRBP1 regulation of granulosa and luteal cell viability. Biology Reproduction, 261-270. | |
| 15640522 | Hnasko, R., et al. 2005. Developmental regulation of PV-1 in rat lung: association with the nuclear envelope and limited colocalization with Cav-1. American Journal of Physiology, L275-284. | |
| 15579465 | Bhattacharya, S.K., et al. 2005. Proteomics reveal Cochlin deposits associated with glaucomatous trabecular meshwork. Journal of Biological Chemistry, 6080-6084. | |
| 15501225 | Olguin, H.C., et al. 2004. Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal. Developmental Biology, 375-388. | |
| 15371336 | Cornelison, D.D.W., et al. 2004. Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration. Genes and Development, 2231-2236. | |
| 15265785 | Gwynn, B., et al. 2004. Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex. Blood, 3181-3189. | |
| 14988380 | Peluso, J.J., et al. 2004. Involvement of an unnamed protein, RDA288, in the mechanism through which progesterone mediates its antiapoptotic action in spontaneously immortalized …. Endocrinology, 3014-3022. | |
| 14724813 | Hase, K., et al. 2003. Expression of LL-37 by human gastric epithelial cells as a potential host defense mechanism against Helicobacter pylori. Gastroenterology, 1613-1625. | |
| 12958441 | Matson, C.T., et al. 2003. Immunoblot detection of brain vascular proteins. Methods in Molecular Medicine, 479-487. | |
| 12805460 | Bjerke, S.L., et al. 2003. Effects of Charged Cluster Mutations on the Function of Herpes Simplex Virus Type 1 UL34 Protein. Journal of Virology, 7601-7610. | |
| 11356697 | Jeffs, B., et al. 2001. Sertoli Cell-Specific Rescue of Fertility, But Not Testicular Pathology, in Dax1 (Ahch)-Deficient Male Mice. Endocrinology, 2481-2488. | |
| 10677043 | Li, H.S., et al. 1999. Activation of a TRPC3-dependent cation current through the neurotrophin BDNF. Neuron, 261-273. | |
| 10362594 | Raman, N., et al. 1999. Rho controls actin cytoskeletal assembly in renal epithelial cells during ATP depletion and recovery. American Journal of Physiology, C1312-C1324. | |