Unlabeled, affinity-purified chicken anti-C-MYC epitope antibody
Volume: 1 mL
Concentration: 100 µg/mL (based on absorbance at 280 nm)
Form: Affinity Purified
Host Species: Chicken
Applications: ELISA, ICC, IHC, IP, WB
Antibody Registry ID (RRID): AB_2313514
Physical State: Liquid
Buffer: Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) as an anti-microbial agent.
Production Notes: Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).
Validation and Application Notes
Western Blot Dilution Range: 1:500-1:1000
IHC Dilution Range: 1:100-1:250
Antibodies were analyzed using ELISA and Western Blots using an N-terminal C-MYC-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.