A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.

A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.

CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.

CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.

Anti-CNPase (cyclic nucleotide phosphodiesterase) (CNP)

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Details

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the CNPase gene product shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: 2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, EC 3.1.4.37

Gene ID: CNP

Antibody Registry ID (RRID): AB_2313538

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 47 kDa

Western Blot Dilution Range: 1:500-1:1000

IHC Dilution Range: 1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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Citations


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