Co-staining of Coronin1 (COR, GREEN, 1:1000 dilution) and Internexin (rabbit antibody, RED, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde.  Photo by Dr. Gerry Shaw, Univ. Florida.  Secondary antibodies (i.e., anti-chicken IgY and anti-rabbit IgG were purchased from Jackson Immunoresearch).

Co-staining of Coronin1 (COR, GREEN, 1:1000 dilution) and Internexin (rabbit antibody, RED, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida. Secondary antibodies (i.e., anti-chicken IgY and anti-rabbit IgG were purchased from Jackson Immunoresearch).

Co-staining of Coronin1 (COR, GREEN, 1:1000 dilution) and Internexin (rabbit antibody, RED, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde.  Photo by Dr. Gerry Shaw, Univ. Florida.  Secondary antibodies (i.e., anti-chicken IgY and anti-rabbit IgG were purchased from Jackson Immunoresearch).

Co-staining of Coronin1 (COR, GREEN, 1:1000 dilution) and Internexin (rabbit antibody, RED, 1:500) in a culture of mouse cerebral cortical tissue fixed with 4% paraformaldehyde. Photo by Dr. Gerry Shaw, Univ. Florida. Secondary antibodies (i.e., anti-chicken IgY and anti-rabbit IgG were purchased from Jackson Immunoresearch).

Anti-Coronin 1A

Anti-Coronin 1A

Anti-Coronin 1A (COR)

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Product Details


Details

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a sequence shared between the human (NM_007074) and mouse (NM_009898) gene products. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Coronin-1A (Coronin-like protein A) (Clipin-A) (Coronin-like protein p57) (Tryptophan aspartate-containing coat protein) (TACO)

Gene ID: CORO1A CORO1

Antibody Registry ID (RRID): AB_2313539

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 51 kDa

Western Blot Dilution Range: 1:5000-1:10000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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