Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against phosphate-buffered isotonic saline. Sodium azide was added, the protein concentration was adjusted to 100 mg/mL, and the antibodies were filter-sterilized (0.2 µm).
Volume: 1 mL
Concentration: 100 µg/mL
Form: Affinity Purified
Host Species: Chicken
Applications: ELISA, ICC, IHC, IP, WB
Antibody Registry ID (RRID): AB_2313510
Physical State: Liquid
Validation and Application Notes
Western Blot Dilution Range: 1:1000-1:2000
IHC Dilution Range: 1:500-1:1000
Antibodies were analyzed using ELISA and western blots using an N-terminal DYKDDDDK-tagged protein. Secondary antibodies used in these quality control assays was an HRP-labeled (1:5000 dilution) goat anti-chicken IgY antibodies (Aves Labs).
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.