Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Chickens were immunized with purified, non-denatured mouse IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-mouse IgG’s were affinity-purified over a column conjugated with purified mouse IgG (containing both heavy and light chains) and then conjugated with fluorescein isothiocyanate.
Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent).
There is no detectable cross-reactivity with rabbit, goat or human IgG
Antibodies were tested for specificity using double-immunodiffusion in agarose and for non-specific binding using cryostat sections of unfixed embryonic chick tissues.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.