Glial Fibrillary Acidic Protein (GFAP) immunostaining (RED) of mixed cortical mouse brain cultures from Gerry Shaw's EnCorBio (Aves Labs, 1:500 dilution). Secondary antibody (Texas Red goat anti-chicken IgY) is from Jackson ImmunoResearch). The BLUE is DAPI staining of nuclei of all cells, including non-GFAP-positive cells.
Immunohistochemical staining of GFAP-positive radial glial cells in the periventricular zone of an e16 mouse brain. Section was a vibratome, thick section (20 um) using a lightly-fixed (2% paraformaldehyde) mouse brain. 1˚ antibody (GFAP) was used at 1:500 dilution; 2˚ antibody (HRP-goat anti-chicken IgY) was used at 1:500 dilution.
Chicken anti-Myelin Basic Protein (MBP) (Green) and Mouse anti-GFAP (Red) with nuclei stained with Hoechst dye (Blue).
Chickens were immunized with recombinant GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.
Volume: 200 µL
Concentration: 2 mg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Glial fibrillary acidic protein (GFAP)
Gene ID: GFAP
Antibody Registry ID (RRID): AB_2313547
Physical State: Liquid
Validation and Application Notes
Expected Banding Pattern: 50 kDa
Western Blot Dilution Range: 1:1000-1:2000
IHC Dilution Range: 1:500-1:1000
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
- Yamada H, Ito D, Oki Y, Kitagawa M, Matsumoto T, Watari T, Kano K (2014), 'Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice.' Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2014.10.082.
- Hasegawa Y, Yoshida D, Nakamura Y, Sakakibara S (2014), 'Spatiotemporal Distribution of SUMOylation Components during Mouse Brain Development.' The Journal of Comparative Neurology Research in Systems Neuroscience. 10.1002/cne.23563.
- Lassiter CM, Gal JS, Becker S, Hartman NW, Grabel L (2016), 'Embryonic stem cell-derived neural progenitors transplanted to the hippocampus migrate on host vasculature.' Stem Cell Research. 10.1016/j.scr.2016.02.043.
- Hegde S, Capell WR, Ibrahim BA, Klett J, Patel NS, Sougiannis AT, Kelly MP (2016), 'Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice.' Neuropsychopharmacology. 10.1038/npp.2016.106.
- Platt RJ, Zhou Y, Slaymaker IM, Shetty AS, Weisbach NR, Kim JA, Sharma J, Desai M, Sood S, Kempton HR, Crabtree GR, Feng G, Zhang F (2017), 'Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits.' Cell Reports. 10.1016/j.celrep.2017.03.052.
- Szot P, Franklin A, Figlewicz DP, Beuca TP, Bullock K, Hansen K, Banks WA, Raskind MA, Peskind ER (2017), 'Multiple lipopolysaccharide (LPS) injections alters interleukin 6 (IL-6), IL-7, IL-10 and IL-6 and IL-7 receptor mRNA in CNS and spleen.' Neuroscience. 10.1016/j.neuroscience.2017.04.028.
- Nagahara AH, Wilson BR, Ivasyk I, Kovacs I, Rawalji S, Bringas JR, Pivirotto PJ, Sebastian WS, Samaranch L, Bankiewicz KS, Tuszynski MH (2017), 'MR-guided delivery of AAV2-BDNF into the entorhinal cortex of non- human primates.' Gene Therapy. 10.1038/s41434-018-0010-2.
- Nakamuta S, Yang YT, Wang CL, Gallo NB, Yu JR, Tai Y, Van Aelst L (2017), 'Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain.' Journal of Cell Biology. 10.1083/jcb.201704157.
- Ronca SE, Smith J, Koma T, Miller MM, Yun N, Dineley KT, Paessler S (2017), 'Mouse Model of Neurological Complications Resulting from Encephalitic Alphavirus Infection.' Frontiers in Microbiology. 10.3389/fmicb.2017.00188.
- Lin JP, Mironova YA, Shrager P, Giger RJ (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.
- Schweig JE, Yao H, Beaulieu-Abdelahad D, Ait-Ghezala G, Mouzon B, Crawford F, Mullan M, Paris D (2017), 'Alzheimer’s disease pathological lesions activate the spleen tyrosine kinase.' Acta Neuropathologica Communications. 10.1186/s40478-017-0472-2.
- Patel NS, Klett J, Pilarzyk K, Lee DI, Kass D, Menniti FS, Kelly MP (2018), 'Identification of new phosphodiesterase 9A (PDE9A) isoforms and how their expression and subcellular compartmentalization in brain changes across the lifespan.' Neurobiology of Aging. 10.1016/j.neurobiolaging.2018.01.019.
- Yu Q, Xue Y, Liu J, Xi Z, Li Z, Liu Y (2018), 'Fibronectin Promotes the Malignancy of Glioma Stem-Like Cells Via Modulation of Cell Adhesion, Differentiation, Proliferation and Chemoresistance.' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2018.00130.
- Yu, FPS; Dworski, S; Medin, JA (2018), 'Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency.' Scientific Reports. 10.1038/s41598-018-20052-6.
- Lo MC, Wang S, Singh S, Damodaran VB, Ahmed I, Coffey K, Barker D, Saste K, Kals K, Kaplan HM, Kohn J, Shreiber DI, Zahn JD (2018), 'Evaluating the in vivo glial response to miniaturized parylene cortical probes coated with an ultra-fast degrading polymer to aid insertion.' Journal of Neural Engineering. 10.1088/1741-2552/aa9fad.
- Zhang Y, Bao Y, Qiu W, Peng L, Fang L, Xu Y, Yang H (2018), 'Structural and visual functional deficits in a rat model of neuromyelitis optica spectrum disorders related optic neuritis.' Experimental Eye Research. 10.1016/j.exer.2018.06.011.
- Mehta SR, Tom CM, Wang Y, Bresee C, Rushton D, Mathkar PP, Tang J, Mattis VB (2018), 'Human Huntington’s Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.' Cell Reports. 10.1016/j.celrep.2018.09.076.
- Morin A, Mouzon B, Ferguson S, Paris D, Saltiel N, Lungmus C, Mullan M, Crawford F (2018), 'Treatment With Nilvadipine Mitigates Inflammatory Pathology and Improves Spatial Memory in Aged hTau Mice After Repetitive Mild TBI.' Frontiers in Aging Neuroscience. 10.3389/fnagi.2018.00292].
- Putatunda R, Zhang Y, Li F, Yang XF, Barbe MF, Hu W (2018), 'Adult neurogenic deficits in HIV-1 Tg26 transgenic mice.' Journal of Neuroinflammation. 10.1186/s12974-018-1322-2.
- Hill JD, Zuluaga-Ramirez V, Gajghate S, Winfield M, Sriram U, Rom S, Persidsky Y (2018), 'Activation of GPR55 induces neuroprotection of hippocampal neurogenesis and immune responses of neural stem cells following chronic, systemic inflammation.' Brain, Behavior, and Immunity. 10.1016/j.bbi.2018.11.017.
- Zhang B, Bailey WM, McVicar AL, Stewart AN, Veldhorst AK, Gensel JC (2018), 'Reducing age-dependent monocyte-derived macrophage activation contributes to the therapeutic efficacy of NADPH oxidase inhibition in spinal cord injury.' Brain, Behavior, and Immunity. 10.1016/j.bbi.2018.11.013.
- Courtney M. Dumont, Mitchell A. Carlson, Mary K. Munsell, Andrew J. Ciciriello, Katerina Strnadova, Jonghyuck Park, Brian J. Cummings, Aileen J. Anderson, Lonnie D. Shea (2018), 'Aligned hydrogel tubes guide regeneration following spinal cord injury.' Acta Biomaterialia. 10.1016/j.actbio.2018.12.052.
- Yiqin Bao, Feng Liu, Xiaobin Liu, Mi Huang, Liumin He, Seeram Ramakrishna, Huanmin Luo, Huiling Hu, Hongying Li, Ying Xu (2019), 'Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways.' Experimental Eye Research. 10.1016/j.exer.2019.01.010.
- Julia TCW, Minghui Wang, Anna A. Pimenova, Kathryn R. Bowles, Brigham J. Hartley, Emre Lacin, Saima I. Machlovi, Rawan Abdelaal, Celeste M. Karch, Hemali Phatnani,Paul A. Slesinger, Bin Zhang, Alison M. Goate, and Kristen J. Brennand (2017), 'An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells.' Stem Cell Reports. 10.1016/j.stemcr.2017.06.018.
- Julia TCW, Claudia M.B. Carvalho, Bo Yuan, Shen Gu, Alyssa N. Altheimer, Shane McCarthy, Dheeraj Malhotra, Jonathan Sebat, Arthur J. Siegel, Uwe Rudolph, James R. Lupski,Deborah L. Levy, and Kristen J. Brennand (2017), 'Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.' Stem Cell Reports. 10.1016/j.stemcr.2017.01.010.
- Fabian PS. Yu, Ben Sajdak, Jakub Sikora, Alexander E. Salmon, Murtaza S. Nagree, Jir?i´ Gurka, Iris S. Kassem, Daniel M. Lipinski, Joseph Carroll, Jeffrey A. Medin (2019), 'Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment.' The American Journal of Pathology. 10.1016/j.ajpath.2018.10.018.
- Meng-chen Lo , Shuwu Wang, Sagar Singh, Vinod B Damodaran, Ijaz Ahmed, Kevin Coffey, David Barker, Kshitij Saste, Karanvir Kals, Hilton M Kaplan, Joachim Kohn, David I Shreiber and Jeffrey D Zahn (2018), 'Evaluating the in vivo glial response to miniaturized parylene cortical probes coated with an ultra-fast degrading polymer to aid insertion.' Journal of Neural Engineering. 10.1088/1741-2552/aa9fad.
- Seok-Man Ho, Brigham J. Hartley, Erin Flaherty, Prashanth Rajarajan, Rawan Abdelaal, Ifeanyi Obiorah, Natalie Barretto, Hamza Muhammad, Hemali P. Phatnani,Schahram Akbarian, and Kristen J. Brennand (2017), 'Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.' Stem Cell Reports. 10.1016/j.stemcr.2017.06.012.
- S. T. Peruzzaro, M. M. M. Andrews, A. Al-Gharaibeh, O. Pupiec, M. Resk, D. Story, P. Maiti, J. RossignolEmail author and G. L. Dunbar (2019), 'Transplantation of mesenchymal stem cells genetically engineered to overexpress interleukin-10 promotes alternative inflammatory response in rat model of traumatic brain injury.' Journal of Neuroinflammation. 10.1186/s12974-018-1383-2.
- A Alachkar, L Wang, R Yoshimura, AR Hamzeh, Z Wang, N Sanathara, SM Lee, X Xu, GW Abbott, and O Civelli (2017), 'Prenatal one-carbon metabolism dysregulation programs schizophrenia-like deficits.' Molecular Psychiatry. 10.1038/mp.2017.164.
- Chandler L. Walker, Colin M.E. Fry, Junmei Wang, Xiaolong Du, Kirstin Zuzzio, Nai-Kui Liu, Melissa J. Walker, and Xiao-Ming Xu (2019), 'Functional and Histological Gender Comparison of Age-Matched Rats after Moderate Thoracic Contusive Spinal Cord Injury.' Journal of Neurotrauma. 10.1089/neu.2018.6233.
- Xiao Xiao, Raj Putatunda, Yonggang Zhang, Priya V. Soni, Fang Li, Ting Zhang, Mingyang Xin, Jin Jun Luo, John R. Bethea, Yuan ChengEmail author and Wenhui Hu (2018), 'Lymphotoxin ß receptor-mediated NF?B signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells.' Journal of Neuroinflammation. 10.1186/s12974-018-1074-z.