Volume: 1 mL
Concentration: 1 mg/mL
Form: Affinity Purified
Host Species: Chicken
Species Reactivity: Rabbit
Physical State: Liquid
Buffer: HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), thimerosal (0.01%, w/v) (as an anti-microbial agent).
Specificity: There is no detectable cross-reactivity with goat, human or mouse IgG.
Production Notes: Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.
Antibodies were tested for specificity using double-immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1:20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.