Low-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).
Higher-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).
Frame showing MAC-1 immunoreactivity in human microglial cells in an Alzheimer's Disease patient. Photomicrograph taken by Dr. Randy Woltjer, Pathology, OHSU.
Our anti-Integrin Alpha-M (MAC-1) antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001076429.1) and human gene products (NP_001139280.1). These antibodies have been validated with human, mouse and rat tissues.
Volume: 1000 µL
Concentration: 100 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: IHC, WB
Protein Name / Synonyms: Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)
Target Description: Integrin Alpha M (also known as MAC-1 and CD11b) is a 1142 amino acid protein expressed in various antigen-presenting cells of the immune system, including microglial cells of the central nervous system. This is the same protein that is recognized by the Ox-42 mouse monoclonal antibody.
Gene ID: ITGAM CD11B CR3A
Antibody Registry ID (RRID): AB_2313548
Physical State: Liquid
Production Notes: Hens were immunized with a Keyhole Limpet Hemocyanin (KLH)-conjugate of a synthetic peptide corresponding to a region near the center of the extracellular domain of this single-membrane pass gene product). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 µg/mL and filter-sterilized.
Validation and Application Notes
Molecular Weight: 170 kDa
Western Blot Dilution Range: 1:2000-1:5000
IHC Dilution Range: 1:2000-1:5000
Antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
- Merlo S, Spampinato SF, Beneventano M, Sortino MA. (2018), 'The contribution of microglia to early synaptic compensatory responses that precede β-amyloid-induced neuronal death.' Nature. 10.1038/s41598-018-25453-1.