MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain. MAP-2 is seen in GREEN (1:1000 dilution); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs, 1:500 dilution). BLUE staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Volume: 1000 µL
Concentration: 200 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Microtubule-associated protein 2 (MAP-2)
Gene ID: MAP2
Antibody Registry ID (RRID): AB_2313549
Physical State: Liquid
Validation and Application Notes
Expected Banding Pattern: 280 kDa
Western Blot Dilution Range: 1:5000-1:10000
IHC Dilution Range: 1:1000-1:2000
Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
- Shahsavani M, Pronk RJ, Falk R, Lam M, Moslem M, Linker SB, Salma J, Day K, Schuster J, Anderlid BM, Dahl N, Gage FH, Falk A. (2017), 'An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis.' Molecular Psychiatry. 10.1038/mp.2017.175.
- Zhang S, Zhao E, Winkelstein BA. (2017), 'A Nociceptive Role for Integrin Signaling in Pain After Mechanical Injury to the Spinal Facet Capsular Ligament.' Annals of Biomedical Engineering. 10.1007/s10439-017-1917-2.
- Niere F, Raab-Graham KF. (2017), 'mTORC1 Is a Local, Postsynaptic Voltage Sensor Regulated by Positive and Negative Feedback Pathways.' Frontiers in Cellular Neuoroscience. 10.3389/fncel.2017.00152.
- Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W. (2017), 'Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.' Neuron. 10.1016/j.neuron.2017.10.003.
- Ash PEA, Dhawan U, Boudeau S, Lei S, Carlomagno Y, Knobel M, Al Mohanna LFA, Boomhower SR, Newland MC, Sherr DH, Wolozin B. (2018), 'Heavy metal neurotoxicants induce ALS-linked TDP-43 pathology.' Toxicological Sciences. 10.1093/toxsci/kfy267.