Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, GREEN, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, RED; 1:1000 dilution). Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, GREEN, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, RED; 1:1000 dilution). Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.

MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.

Chicken anti-Myelin Basic Protein (MBP) (Green) and Mouse anti-GFAP (Red) with nuclei stained with Hoechst dye (Blue).

Chicken anti-Myelin Basic Protein (MBP) (Green) and Mouse anti-GFAP (Red) with nuclei stained with Hoechst dye (Blue).

from Aves Labs

Anti-Myelin Basic Protein Antibody (MBP)

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Product Details | ↓ Citations


An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.


Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)

Target Description: Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.

Gene ID: MBP

Antibody Registry ID (RRID): AB_2313550

Physical State: Liquid

Production Notes: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Validation and Application Notes

Molecular Weight: Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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