Anti-Myelin Basic Protein Antibody

29 Citations

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.

Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.
Immunoblot images of MAG and MBP (cat. MBP, 1:2000) in the somatosensory cortex of wildtype and GluD1 KO. Significant increase in MAG (***p < 0.0001, unpaired t-test) and MBP (*p < 0.0104, unpaired t-test) was found in GluD1 KO mice compared to wildtype. Image from publication CC-BY-4.0. PMID:37983226
Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, green, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, red; 1:1000 dilution). Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.
Immunohistochemical staining of the MBP (green) in the nodes of Ranvier of the sciatic nerve of an adult rat. Red counterstaining is a ubiquitous gene product. Blue shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.
Immunolabeling of MBP (red, 1:200), CD45 (green, 1:100), and DAPI (1:1000, nuclei) of a mouse spinal cord lysophosphatidylcholine lesion. Image kindly provided by Charbel Baaklini, Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Canada
Immunostaining in the cortical and hippocampal region of paraffin embedded transgenic adult mouse brain identifying MBP (Cat no MBP, 1:1000, red) with nuclear staining with DAPI. Images taken with an epifluorescence microscope, 10x magnification. Image kindly provided by Begüm Kurt, Achucarro Basque Center for Neuroscience.
Immunohistochemical staining of the MBP (green) in the nodes of Ranvier of the sciatic nerve of an adult rat. Red counterstaining is a ubiquitous gene product. Blue shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.
Chicken anti-Myelin Basic Protein (MBP) (green), with nuclei stained with Hoechst dye (blue).
Representative images passing through corpus callosum showing MBP (cat. MBP 1:250; red) immunoreactivity in wildtype and GluD1 KO mice. A significant reduction in MBP staining was observed in GluD1 KO mice compared to wildtype following cuprizone treatmentImmunostaining in mouse corpus callosum in wild type and GluD1 knockouts labeling MBP. Image from publication CC-BY-4.0. PMID:37983226
MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.
Representative images are for myelin basic protein, MBP (cat. MBP 1:250; red), staining in corpus callosum and motor cortex of wildtype and GluD1 KO mice at P40 and P180 and its quantification. GluD1 KO mice showed increased MBP staining in corpus callosum (*p = 0.0196, unpaired t-test) and motor cortex (****p < 0.0001, unpaired t-test) at P40. Image from publication CC-BY-4.0. PMID:37983226
Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.
Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.
Immunoblot images of MAG and MBP (cat. MBP, 1:2000) in the somatosensory cortex of wildtype and GluD1 KO. Significant increase in MAG (***p < 0.0001, unpaired t-test) and MBP (*p < 0.0104, unpaired t-test) was found in GluD1 KO mice compared to wildtype. Image from publication CC-BY-4.0. PMID:37983226
Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, green, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, red; 1:1000 dilution). Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.
Immunohistochemical staining of the MBP (green) in the nodes of Ranvier of the sciatic nerve of an adult rat. Red counterstaining is a ubiquitous gene product. Blue shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.
Immunolabeling of MBP (red, 1:200), CD45 (green, 1:100), and DAPI (1:1000, nuclei) of a mouse spinal cord lysophosphatidylcholine lesion. Image kindly provided by Charbel Baaklini, Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Canada
Immunostaining in the cortical and hippocampal region of paraffin embedded transgenic adult mouse brain identifying MBP (Cat no MBP, 1:1000, red) with nuclear staining with DAPI. Images taken with an epifluorescence microscope, 10x magnification. Image kindly provided by Begüm Kurt, Achucarro Basque Center for Neuroscience.
Immunohistochemical staining of the MBP (green) in the nodes of Ranvier of the sciatic nerve of an adult rat. Red counterstaining is a ubiquitous gene product. Blue shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.
Chicken anti-Myelin Basic Protein (MBP) (green), with nuclei stained with Hoechst dye (blue).
Representative images passing through corpus callosum showing MBP (cat. MBP 1:250; red) immunoreactivity in wildtype and GluD1 KO mice. A significant reduction in MBP staining was observed in GluD1 KO mice compared to wildtype following cuprizone treatmentImmunostaining in mouse corpus callosum in wild type and GluD1 knockouts labeling MBP. Image from publication CC-BY-4.0. PMID:37983226
MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.
Representative images are for myelin basic protein, MBP (cat. MBP 1:250; red), staining in corpus callosum and motor cortex of wildtype and GluD1 KO mice at P40 and P180 and its quantification. GluD1 KO mice showed increased MBP staining in corpus callosum (*p = 0.0196, unpaired t-test) and motor cortex (****p < 0.0001, unpaired t-test) at P40. Image from publication CC-BY-4.0. PMID:37983226
Human, Mouse, Rabbit, Rat, Sheep
ICC, IHC, WB
Chicken

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SKU: MBP

Volume: 1000 µL
Price:
$455

Product Specific References for Applications and Species

Immunocytochemistry: Rabbit
PMID Dilution Publication
353436551:100Liu, P., et al. 2022. A comparative study of bronchopulmonary slowly adapting receptors between rabbits and rats. Physiological reports, e15069.
Immunocytochemistry: Rat
PMID Dilution Publication
372727181:500Nakamura, DS, et al. 2023. Expression and Subcellular Localization of Mitochondrial Docking Protein, Syntaphilin, in Oligodendrocytes and CNS Myelin Sheath. Glia, .
353436551:100Liu, P., et al. 2022. A comparative study of bronchopulmonary slowly adapting receptors between rabbits and rats. Physiological reports, e15069.
32910475not listedNakamura, D.S., et al. 2020. Mitochondrial Dynamics and Bioenergetics Regulated by Netrin-1 in Oligodendrocytes.. Glia, 392-412.
309373981:1000Xu, Y.K.T., et al. 2019. Deep learning for high-throughput quantification of oligodendrocyte ensheathment at single-cell resolution. Communications Biology, 116.
Immunohistochemistry: Mouse
PMID Dilution Publication
381003561:200Baaklini, CS, et al. 2023. Microglia promote remyelination independent of their role in clearing myelin debris. Cell Reports, 113574.
379832261:250Gakare, SG, et al. 2023. Glutamate delta-1 receptor regulates oligodendrocyte progenitor cell differentiation and myelination in normal and demyelinating conditions. PloS one, e0294583.
363436781:300Ghalat, J., et al. 2023. Cis-p-tau Plays Crucial Role In Lysolecithin-Induced Demyelination And Subsequent Axonopathy In Mouse Optic Chiasm. Experimental Neurology, 114262.
360899141:500Luo, W., et al. 2022. Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation. Glia, 284-304.
343629121:1000Call, C.L., et al. 2021. Cortical neurons exhibit diverse myelination patterns that scale between mouse brain regions and regenerate after demyelination. Nature Communications, 4767.
311169731:100Xin, W., et al. 2019. Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase.. Cell Reports, 2262-2271.
308437561:500Meyer, C.E., et al. 2019. Axonal damage in spinal cord is associated with gray matter atrophy in sensorimotor cortex in experimental autoimmune encephalomyelitis. Multiple Sclerosis Journal, 294-303.
304061831:200Micheva, K.D., et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, .
295960471:500Larson, V.A., et al. 2018. Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility. eLife, e34829.
294044021:500Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, .
288212761:500Santo Araújo, S.E., et al. 2017. Inflammatory demyelination alters subcortical visual circuits. Jounral of Neuroinflammation, 162.
287608621:500Assinck, P., et al. 2017. Myelinogenic Plasticity of Oligodendrocyte Precursor Cells following Spinal Cord Contusion Injury. The Journal of Neuroscience, 8635-8654.
273393931:200Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice. Neuropsychopharmacology, 2920-2931.
254512511:1000Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346.
Immunohistochemistry: Rabbit
PMID Dilution Publication
348488031:100Liu, J., et al. 2021. A single baroreceptor unit consists of multiple sensors. Scientific Reports, 23111.
280182311:2000Liu, J., et al. 2016. Slowly Adapting Sensory Units Have More Receptors in Large Airways than in Small Airways in Rabbits. Frontiers in Pysciology, 588.
Immunohistochemistry: Rat
PMID Dilution Publication
330061241:300Moreno-Luna, R., et al. 2020. Heterogeneity of the Endocannabinoid System Between Cerebral Cortex and Spinal Cord Oligodendrocytes. Molecular Neurobiology, 689-702.
304097581:200Yung, A., et al. 2018. Diffusion tensor imaging shows mechanism-specific differences in injury pattern and progression in rat models of acute spinal cord injury. Neuroimage, 43-55.
Immunohistochemistry: Sheep
PMID Dilution Publication
305232731:500Fowke, T.M., et al. 2018. Loss of interneurons and disruption of perineuronal nets in the cerebral cortex following hypoxia-ischaemia in near-term fetal sheep. Scientific Reports, 17686.
Western Blot: Human
PMID Dilution Publication
35806400not listedKamaeva, D.A., et al. 2022. Catalytic Antibodies in Bipolar Disorder: Serum IgGs Hydrolyze Myelin Basic Protein. International journal of molecular sciences, 7397.
Western Blot: Mouse
PMID Dilution Publication
379832261:2000Gakare, SG, et al. 2023. Glutamate delta-1 receptor regulates oligodendrocyte progenitor cell differentiation and myelination in normal and demyelinating conditions. PloS one, e0294583.
305716671:2000Xu, Z., et al. 2018. Arecoline attenuates memory impairment and demyelination in a cuprizone-induced mouse model of schizophrenia. Neuroreport, 134-138.
284840081:200Beirowski, B., et al. 2017. mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells. PNAS, E4261-E4270.
28407788not listedChoquet, K., et al. 2017. Absence of neurological abnormalities in mice homozygous for the Polr3a G672E. Molecular Brain, 13.
Additional Publications: Unspecified
PMID Publication
35568187Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114.

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