Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain.  RED shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500).  Green is staining of the granule cells; BLUE is DAPI nuclear staining.  Page Balich, Univ. Arizona.

Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. RED shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; BLUE is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of 3T3 cells in culture.  Nestin immunoreactivity (RED staining, Aves Labs, 1:1000 dilution; GREEN is -Tubulin 3 staining using a rabbit antibody (Rockland, 1:500 dilution); BLUE is DAPI nuclear staining.  Page Balich, Univ. Arizona.

Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (RED staining, Aves Labs, 1:1000 dilution; GREEN is -Tubulin 3 staining using a rabbit antibody (Rockland, 1:500 dilution); BLUE is DAPI nuclear staining. Page Balich, Univ. Arizona.

Immunocytochemistry showing nestin immunoreactivity (GREEN; 1:1000 dilution) in A7r5 neuroblastoma cells in culture.  BLUE is DAPI nuclear staining.  Page Balich, Univ. Arizona.

Immunocytochemistry showing nestin immunoreactivity (GREEN; 1:1000 dilution) in A7r5 neuroblastoma cells in culture. BLUE is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of A7r5 neuroblastoma cells in culture.  Nestin immunoreactivity (RED staining, Aves Labs, 1:1000 dilution; GREEN is -Tubulin 3 staining using a rabbit antibody (Rockland, 1:500 dilution); BLUE is DAPI nuclear staining.  Page Balich, Univ. Arizona.

Photomicrograph of A7r5 neuroblastoma cells in culture. Nestin immunoreactivity (RED staining, Aves Labs, 1:1000 dilution; GREEN is -Tubulin 3 staining using a rabbit antibody (Rockland, 1:500 dilution); BLUE is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed).  GREEN is nestin immunoreactivity (Aves Labs, 1:1000 dilution).  BLUE is DAPI nuclear staining.  Hoda Ilias, Aves Labs.

Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed). GREEN is nestin immunoreactivity (Aves Labs, 1:1000 dilution). BLUE is DAPI nuclear staining. Hoda Ilias, Aves Labs.

"Nestin Western Blot.  20 ug of total protein from adult mouse brain in each lane. Lane A is Aves Labs' antibody (1:2000), showing a single, strong band at the correct molecular weight. Lane B uses a rabbit antibody from BD Transduction Labs showing a weak band at the same molecular weight, as well as another spurious band at about 145 kDa. Hoda Ilias, Aves Labs."

"Nestin Western Blot. 20 ug of total protein from adult mouse brain in each lane. Lane A is Aves Labs' antibody (1:2000), showing a single, strong band at the correct molecular weight. Lane B uses a rabbit antibody from BD Transduction Labs showing a weak band at the same molecular weight, as well as another spurious band at about 145 kDa. Hoda Ilias, Aves Labs."

Anti-Nestin (NES)

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Details

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Volume: 1000 µL

Concentration: 300 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Nestin

Gene ID: NES Nbla00170

Antibody Registry ID (RRID): AB_2314882

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 250 kDa

Western Blot Dilution Range: 1:10000-1:20000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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Citations


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