Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (Aves Labs Cat.# NFH; 1:5000 dilution).  Secondary antibody (fluorescein-labeled goat anti-chicken IgY) from Jackson Immunoresearch (1:1000 dilution)

Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (Aves Labs Cat.# NFH; 1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY) from Jackson Immunoresearch (1:1000 dilution)

Anti-NF-H (Neurofilament 200 kDa) (NFH)

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Details

Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Volume: 200 µL

Concentration: 1 mg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)

Gene ID: NEFH KIAA0845 NFH

Antibody Registry ID (RRID): AB_2313552

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 200-220 kDa

Western Blot Dilution Range: 1:2000-1:5000

IHC Dilution Range: 1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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Citations


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