Eggs from at least 20 chickens were purified from the yolks using a proprietary method that yields a purity of >90%. The IgY fraction was then dialyzed against phosphate-buffered isotonic saline. Sodium azide was then added and the preparation was filter-sterilized (0.45 μm filter).
Volume: 1 mL
Physical State: Liquid
Buffer: Phosphate-buffered saline (PBS)(pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent).
Antibodies were analyzed by SDS-polyacrylamide gel electrophoresis. Heavy chains (80 kDa), truncated heavy chains (40 kDa) and light chains (25 kDa) were found to make up >95% of the Coomassie-stainable material.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.