Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse.  (GREEN) P0 concentration was a 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution).  RED is actin.  BLUE is DAPI nuclear counterstain.  Photomicrograph from Dr. Gerry Shaw, EnCor Biotechnology.

Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (GREEN) P0 concentration was a 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). RED is actin. BLUE is DAPI nuclear counterstain. Photomicrograph from Dr. Gerry Shaw, EnCor Biotechnology.

Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse.  P0 concentration was a 1:1000 dilution, followed by HRP-labeled goat anti-chicken IgY (1:500 dilution).  Hoda Ilias, Aves Labs.

Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. P0 concentration was a 1:1000 dilution, followed by HRP-labeled goat anti-chicken IgY (1:500 dilution). Hoda Ilias, Aves Labs.

Anti-P-Zero Myelin Protein (PZO)

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Details

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 200 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Myelin protein P0 (Myelin peripheral protein) (MPP) (Myelin protein zero)

Gene ID: MPZ

Antibody Registry ID (RRID): AB_2313561

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 27 kDa

Western Blot Dilution Range: 1:10000-1:20000

IHC Dilution Range: 1:2000-1:5000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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