immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.

immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.

Adult rat brain immunohistochemistry.

Adult rat brain immunohistochemistry.

Adult rat brain membrane immunoblot.

Adult rat brain membrane immunoblot.

Pan-Neurofascin (extracellular) (7x-172)

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Product Details | ↓ Citations


Details

Concentration: 1 mg/mL

Clonality: Monoclonal

Form: Purified

Host Species: Mouse

Immunogen: Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin by: - binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186)
Mouse: 98% identity (1074/1086 amino acids identical)
Human: 79% identity (955/1195 amino acids identical)
This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Gene ID: Nfasc

Antibody Registry ID (RRID): AB_2282826

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 155, 186 kDa

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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Citations


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