Double-labeled fluorescence immunohistochemistry of human midbrain dopamine neurons stained green with LNC1 and red with PitX.

Double-labeled fluorescence immunohistochemistry of human midbrain dopamine neurons stained green with LNC1 and red with PitX.

LNC1 anti-TH monoclonal antibody was used at 2.5ug/mL to stain endogenous tyrosine hydroxylase in rat brain lysate.

LNC1 anti-TH monoclonal antibody was used at 2.5ug/mL to stain endogenous tyrosine hydroxylase in rat brain lysate.

Mouse brain midbrain dopamine neurons in the substantia nigra double-labeled with LNC1 for tyrosine hydroxylase (TH, red and cytoplasmic) and a rabbit antibody to Nuclear Factor Y subunit A (NFYA, green and primarily nuclear).

Mouse brain midbrain dopamine neurons in the substantia nigra double-labeled with LNC1 for tyrosine hydroxylase (TH, red and cytoplasmic) and a rabbit antibody to Nuclear Factor Y subunit A (NFYA, green and primarily nuclear).

Western Blot of HEK293 cells transfected to express human Tyrosine Hydroxylase isoform 2 and Green Fluorescent Protein demonstrates the specificity of mouse monoclonal antibody LNC1 to tyrosine hydroxylase protein.

Western Blot of HEK293 cells transfected to express human Tyrosine Hydroxylase isoform 2 and Green Fluorescent Protein demonstrates the specificity of mouse monoclonal antibody LNC1 to tyrosine hydroxylase protein.

Anti-Tyrosine Hydroxylase (LNC1) (76-700-100)

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Details

LNC1 is an antibody which can be used to detect Tyrosine Hydroxylase (TH). TH is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine and is responsible for converting L-tyrosine to L-dopa. Synthesis of catecholamines is regulated by the interaction of TH with its’ cofactor, tetrahydrobiopterin (BH4) and the substrates L-tyrosine and molecular oxygen. In humans four TH mRNA splice variants (hTH1-hTH4) have been isolated while subprimate species rely on a single form of TH.  It is known that the hTH1-hTH4 variants are identical in their catalytic domain but differ in their N-terminal regulatory domains. Importantly, LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH. The role of TH in the synthesis of catecholamine neurotransmitters suggests a connection between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels, such as Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.

Volume: 100 µL, 20 µL

Concentration: 1 mg/mL

Clonality: Monoclonal

Form: Ascites Fluid

Host Species: Mouse

Antibody Isotype: IgG1

Species Reactivity: Chicken, Frog, Human, Lizard, Monkey, Mouse, Rat, Vole, Zebrafish

Applications: ICC, IHC, IP, WB

Immunogen: Tyrosine Hydroxylase purified from PC12 cells.

Protein Name / Synonyms: Tyrosine 3-monooxygenase

Gene ID: TH, TYH, EC 1.14.16.2

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 59-63 kDa

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Storage

Aliquot and store at -20 degrees celcius. Stable for 2 years after opening when stored as described.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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