Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts.  Anti-vimentin antibody was used at a dilution of 1:1000; HRP-labeled goat anti-chicken IgY was used at a dilution of 1:500.

Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody was used at a dilution of 1:1000; HRP-labeled goat anti-chicken IgY was used at a dilution of 1:500.

Composite IHC and ICC photomicrographs.  The IHC panel (left) is the same as Vimentin.2.jpg.  The ICC panel (right) is the same as Vimentin.jpg, below.

Composite IHC and ICC photomicrographs. The IHC panel (left) is the same as Vimentin.2.jpg. The ICC panel (right) is the same as Vimentin.jpg, below.

Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity.  Vimentin antibody was used at a 1:1000 dilution; Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, F-1005) was used at a 1:500 dilution.  Hoda Ilias, Aves Labs.

Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody was used at a 1:1000 dilution; Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, F-1005) was used at a 1:500 dilution. Hoda Ilias, Aves Labs.

Vimentin western blot of a homogenate prepared from a neurosphere culture.  Vimentin antibody was used at a 1:2000 dilution; Secondary, HRP-labeled goat anti-chicken antibody (Aves Labs, H-1004) was used at a 1:1000 dilution.  Hoda Ilias, Aves Labs.

Vimentin western blot of a homogenate prepared from a neurosphere culture. Vimentin antibody was used at a 1:2000 dilution; Secondary, HRP-labeled goat anti-chicken antibody (Aves Labs, H-1004) was used at a 1:1000 dilution. Hoda Ilias, Aves Labs.

Anti-Vimentin protein (VIM)

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Product Details


Details

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the vimentin gene product, but are shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Volume: 1000 µL

Concentration: 300 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Vimentin

Gene ID: VIM

Antibody Registry ID (RRID): AB_2313565

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 52 kDa

Western Blot Dilution Range: 1:2000-1:5000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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