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Western Blotting Using Alkaline Phosphatase-Labeled Secondary
Solutions PBS – sodium phosphate-buffered (100 mM; pH 7.2) isotonic (0.9% NaCl, w/v) saline Antibody dilution buffer (PBS with 0.1% non-ionic detergent, such as Triton...
Western Blotting using HRP-Labeled Secondary
Solutions PBS – sodium phosphate-buffered (100 mM; pH 7.2) isotonic (0.9% NaCl, w/v) saline Antibody dilution buffer (PBS with 0.1% non-ionic detergent, such as Triton...
Immunocytochemistry using Fluorescein-labeled Secondary Antibodies
Solutions PBS – sodium phosphate-buffered (100 mM; pH 7.2) isotonic (0.9% NaCl, w/v) saline Antibody dilution buffer (PBS with 0.1% non-ionic detergent, such as Triton...
Immunoprecipitation
Chicken IgY’s lack the Fc domain where proteins A and G normally bind. Consequently, protein A/G-Agarose or -Sepharose cannot be used to immunoprecipitate chicken IgY’s....
Citrate Buffer Antigen Retrieval Protocol
Background for Citrate Buffer Antigen Retrieval Formaldehyde fixation (2% or 4%, or as a component of 10% formalin) produces protein cross-links in tissues that tend...