Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat# p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser-133), and phosphorylation of Ser-133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
Volume: 100 µL
Concentration: Lot Specific
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Rat
Applications: WB, IHC
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
Protein Name / Synonyms: p-creb, pcreb
Gene ID: CREB1
Antibody Registry ID (RRID): AB_2492066
Physical State: Liquid
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 45
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.