Anti-mCherry Antibody

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1 Citation

Our anti-mCherry chicken primary antibody from AvesLabs is manufactured in-house. It detects mCherry, dsRED, RFP (all tested) and tdTOMATO (by similarity) and is purified. It is provided in liquid form.

Primary Antibody
ICC, IHC, WB
Chicken
Affinity purified
MCHERRY
27 KDa for native monomer

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Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
Western blotting of recombinant fluorescent proteins showing Aves Labs chicken anti-mCherry antibody recognizing nanogram amounts of mCherry protein and the related dsRED protein, but not showing reactivity with GFP.
Western blot of COS7 cells transfected with mCherry-myc-FLAG (lane 1), GFP (lane 2), or mock transfected cells (lane 3) using chicken α-mCherry antibody showing the expected staining for mCherry in lane 1 and no staining in GFP or untransfected cells. The ~37kDa major band in lane 1 represents full-length mCherry-myc-FLAG while the shorter ~24kDa band represents an mCherry degradation product.
IF of a section of mouse brain NAc labeled with Anti-mCherry (1:1000, red), colabeled with eGFP, green, and DAPI staining of nuclear DNA. The Anti-mCherry specifically targeted the cpmApple-based membrane-localized red dopamine sensor co-expressed with eGFP in NAc and identified both cellular and subcellular distribution of the sensor. 20X/0.8 Air & 40X/1.1 Water Confocal, scale bar 100 um; 20 um. Donkey anti-chicken AlexaFluor647. Image from by Zikai Wang, Biomedical Engineering, USC.
Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry (cat. MCHERRY, 1:100; green). Image kindly provided by Erica Yao, University of California San Francisco).
Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry (cat. MCHERRY, 1:100; green). Nkx2.1 (red) marks the epithelium and nuclei are labeled with DAPI (blue). Image kindly provided by Erica Yao, University of California San Francisco).
Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
Western blotting of recombinant fluorescent proteins showing Aves Labs chicken anti-mCherry antibody recognizing nanogram amounts of mCherry protein and the related dsRED protein, but not showing reactivity with GFP.
Western blot of COS7 cells transfected with mCherry-myc-FLAG (lane 1), GFP (lane 2), or mock transfected cells (lane 3) using chicken α-mCherry antibody showing the expected staining for mCherry in lane 1 and no staining in GFP or untransfected cells. The ~37kDa major band in lane 1 represents full-length mCherry-myc-FLAG while the shorter ~24kDa band represents an mCherry degradation product.
IF of a section of mouse brain NAc labeled with Anti-mCherry (1:1000, red), colabeled with eGFP, green, and DAPI staining of nuclear DNA. The Anti-mCherry specifically targeted the cpmApple-based membrane-localized red dopamine sensor co-expressed with eGFP in NAc and identified both cellular and subcellular distribution of the sensor. 20X/0.8 Air & 40X/1.1 Water Confocal, scale bar 100 um; 20 um. Donkey anti-chicken AlexaFluor647. Image from by Zikai Wang, Biomedical Engineering, USC.
Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry (cat. MCHERRY, 1:100; green). Image kindly provided by Erica Yao, University of California San Francisco).
Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry (cat. MCHERRY, 1:100; green). Nkx2.1 (red) marks the epithelium and nuclei are labeled with DAPI (blue). Image kindly provided by Erica Yao, University of California San Francisco).

Anti-mCherry Antibody
SKU: MCHERRY-0100

Volume: 500 µL
Price:
$440

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Product Specific References for Applications and Species

  • Immunocytochemistry: Human
Immunocytochemistry: Human
PMID Dilution Publication
359413691:1000Sahu, S.K., et al. 2022. A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development. Nature Cell Biology, 1265-1277.

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