NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.
- Figlia G, Norrmén C, Pereira JA, Gerber D, Suter U. (2017), 'Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system.' eLife. 10.7554/eLife.29241.
- Arevalo-Martin A, Grassner L, Garcia-Ovejero D, Paniagua-Torija B, Barroso-Garcia G, Arandilla AG, Mach O, Turrero A, Vargas E, Alcobendas M, Rosell C, Alcaraz MA, Ceruelo S, Casado R, Talavera F, Palazón R, Sanchez-Blanco N, Maier D, Esclarin A, Molina-Holgado E. (2018), 'Elevated Autoantibodies in Subacute Human Spinal Cord Injury Are Naturally Occurring Antibodies.' Frontiers in Immunology. 10.3389/fimmu.2018.02365.
- Lee SJ, Oses-Prieto JA, Kawaguchi R, Sahoo PK, Kar AN, Rozenbaum M, Oliver D, Chand S, Ji H, Shtutman M, Miller-Randolph S, Taylor RJ, Fainzilber M, Coppola G, Burlingame AL, Twiss JL. (2018), 'hnRNPs interacting with mRNA localization motifs define axonal RNA regulons .' Molecular & Cellular Proteomics. 10.1074/mcp.RA118.000603.
- Hines TJ, Gao X, Sahu S, Lange MM, Turner JR, Twiss JL, Smith DS. (2018), 'An Essential Postdevelopmental Role for Lis1 in Mice.' eNeuro. 10.1523/ENEURO.0350-17.2018.