Our Anti-S100B chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat S100B, and is affinity-purified IgY. It is great for use in ICC, WB.
Immunofluorescent staining of COS-7 cells expressing S100B-flag using Aves Labs anti-S100B antibody (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.
S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. By contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells. S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells. This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metastasis.
Affinity-Purified IgY
0.2 mg/ml
Polyclonal
IgY
ICC, IHC, WB
Chicken
S100B
14 kDa
His tagged full length protein
Human, Mouse, Rat
AB_2943661
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested in WB to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
S100 Calcium Binding Protein B, S100beta, S-100 Protein Subunit Beta, Protein S100-B, S100 Calcium Binding Protein, Beta (Neural), S100 Calcium-Binding Protein B, S-100 Protein Beta Chain, S100-B, S100, NEF
UniProt (Human): P04271
Bulk Order Anti-S100B Antibody
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