Immunoblot against extracts of COS cells transfected with untagged VGlut or Kv2.1 plasmid and probed with N28/9 (left) or N52A/42 (right) TC supe.
Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles. Isoform 2 is expressed in retinal. Vglut1 functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut1 has been implicated in several neurological and psychiatric diseases including Alzheimer’s disease, Parkinson’s disease, epilepsy and schizophrenia.
ICC, IHC, WB
Slc17a7 Bnpi Vglut1
Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (also known as Vesicular glutamate transporter 1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Bnpi, Solute carrier family 17 member 7 and Slc17a7, accession number Q62634); Mouse: 97% identity (66/68 amino acids identical); Human: 95% identity (65/68 amino acids identical); 50% identity with VGlut2 or VGlut3
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.