Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.
Volume: 200 µL
Concentration: 2 mg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Peripherin (Neurofilament 4)
Target Description: Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
Gene ID: PRPH NEF4 PRPH1
Antibody Registry ID (RRID): AB_2313558
Physical State: Liquid
Production Notes: Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.
Validation and Application Notes
Molecular Weight: 60 kDa
Western Blot Dilution Range: 1:2000-1:5000
IHC Dilution Range: 1:2000-1:5000
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
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